FastQCFastQC Report
Tue 7 May 2024
EGAF00007823370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16647
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTA6253.754430227668649No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTT2901.742055625638253No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2761.6579563885384754No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGA2351.411665765603412No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGT2311.3876374121463328No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1801.081275905568571No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.889049077911936No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.7869285757193488No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTG900.5406379527842855No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTC840.5045954225986664No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA800.48056706914158703No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG630.3784465669489998No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA620.37243947858473No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG590.3544182134919205No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3243827716705713No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC460.2763260647564126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG400.24028353457079352No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG400.24028353457079352No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA380.22826935784225388No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.22826935784225388No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC320.19222682765663485No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.19222682765663485No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC310.186219739292365No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA300.18021265092809513No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.17420556256382533No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA290.17420556256382533No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC260.1561842974710158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC250.15017720910674595No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC250.15017720910674595No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC240.14417012074247612No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGT230.1381630323782063No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA220.13215594401393646No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT220.13215594401393646No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA200.12014176728539676No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA200.12014176728539676No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.11413467892112694No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA180.10812759055685708No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACT180.10812759055685708No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA180.10812759055685708No Hit
CCCTACTAGTCTCACCCCAAGCAGGCTGTCCCATACCCGTGTTGACGCTT170.10212050219258724No Hit
GTCCCATACCCGTGTTGACGCTTTCCGACGTCGACCCCGTCATGGTATCC170.10212050219258724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)