FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102
Sequences flagged as poor quality0
Sequence length151
%GC71

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT16014.519056261343014No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC625.626134301270418No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC302.722323049001815No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC80.7259528130671506Illumina RNA PCR Primer (95% over 23bp)
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG60.5444646098003629No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGG60.5444646098003629No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG50.4537205081669692No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.4537205081669692No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGGGGGGGGGC40.3629764065335753No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGGGGGGGGGG40.3629764065335753No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG40.3629764065335753No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGCGGGGG30.27223230490018147No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.27223230490018147No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTCTCGGGGGGGGGC30.27223230490018147No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGCGGGGGGGGGC30.27223230490018147No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGGGGGG30.27223230490018147No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTGTGGGGGGGGGGG30.27223230490018147No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGGGGG30.27223230490018147No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGG30.27223230490018147No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGGGGGGG30.27223230490018147No Hit
TGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGC20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGTTGTGGGGGGGGGGG20.18148820326678766No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGGGGGGGGGG20.18148820326678766No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTCGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGTGGGG20.18148820326678766No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTTGTCTCGGGGGGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGGGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTCGGTGGGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTGGGGGGGGGGGGGG20.18148820326678766No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACCATCTGACGCTGCC20.18148820326678766No Hit
ATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCCG20.18148820326678766Illumina RNA PCR Primer (95% over 24bp)
GTGTAAAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.18148820326678766No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTGGGGGG20.18148820326678766No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGGGGCGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTATATATCGGTGGTCGCC20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGGGGCGGGGGGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGTGGGGGGG20.18148820326678766No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC20.18148820326678766No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGG20.18148820326678766No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTCGCGGGGGGGGGG20.18148820326678766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)