FastQCFastQC Report
Tue 7 May 2024
EGAF00007823379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10707
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG3343.1194545624357897No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT2332.1761464462501166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1871.7465209675912954No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1631.5223685439432149No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1221.1394414868777436No Hit
ATTGCGCAATGCTTTACGAGGGGTCTTTCCTTTTGCGGCCATCGGTGGAT1060.9900065377790231No Hit
ATTGCGCAATGTCACATCAGGGGAATTCAAGGGTGCGAGGCCAGTCGCTT790.7378350611749322No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC580.5417016904828617No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG570.5323620061641917No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCT480.44830484729616144No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAT450.4202857943401513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC390.36424768842813116No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA360.336228635472121No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT360.336228635472121No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT320.2988698981974409No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT300.2801905295601009No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA280.26151116092276083No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA270.2521714766040908No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG270.2521714766040908No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.18679368637340057No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG180.1681143177360605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC180.1681143177360605No Hit
ATTGCGCAATGAAATCATAGGGGCTTCAAGAAATCTTAACCGTTAAGTCG160.14943494909872046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG160.14943494909872046No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCG150.14009526478005044No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAT150.14009526478005044No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG140.13075558046138042No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCGCG140.13075558046138042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT140.13075558046138042No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCT140.13075558046138042No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAGCG130.12141589614271038No Hit
ATTGCGCAATGGACTAGGCGGGGGCCTGTGGTTCGCAACGTTCTGTCAGC130.12141589614271038No Hit
ATTGCGCAATGACCAGCCGGGGGGGTTGTAAAGCTCTTTTGTCCGGGACG130.12141589614271038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.12141589614271038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA130.12141589614271038No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.12141589614271038No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCT130.12141589614271038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC120.11207621182404036No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG120.11207621182404036No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA110.10273652750537032No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.10273652750537032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.10273652750537032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.10273652750537032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)