FastQCFastQC Report
Tue 7 May 2024
EGAF00007823381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18055
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG6863.799501523123788No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCT5973.306563278870119No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3451.910828025477707No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3401.8831348656881752No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1821.0080310163389643No Hit
ATTGCGCAATGGGCTAAGCGGGGGAGCCCTTCACGGAAGGTGATCGTTGA1790.991415120465245No Hit
ATTGCGCAATGGGATTCCAGGGGAAAGGACATCAACAGAGACTCCCGTAG1480.8197175297701467No Hit
ATTGCGCAATGACGTGTGTGGGATGACGGCCTTCGGGTTGTAAACCGCTT1340.7421766823594572No Hit
ATTGCGCAAGGGACTGACGGGGGACACGGGGCTTGGCTTGGAGGGCCCAG1290.7144835225699252No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGT1020.5649404597064525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG960.5317086679590142No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAAT830.45970645250623093No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC830.45970645250623093No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC690.3821656050955414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.3710883411797286No Hit
ATTGCGCAATGAAGCCCTAGGGTGGTAATAATATAAAGAAAATAATAGCG640.3544724453060094No Hit
ATTGCGCAATGCCTGACTGGGGGAGGCGTCAAGCAGTCGATATTTCTCCT630.34893381334810303No Hit
ATTGCGCAATGGACGGGGCGGGATACCACCTCGCCCGAGCCCATTTCTTT520.28800886181113267No Hit
ATTGCGCAATGTGAGTGACGGGGACACTAAAGACACAAAAAGAATAAATT510.28247022985322623No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG500.27693159789531985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT490.27139296593741347No Hit
ATTGCGCAATGGTCTTCAAGGGGTTATTAAGGGCGCATGGTGGATGCCTT480.2658543339795071No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.2603157020216007No Hit
ATTGCGCAATGGGATATTCGGGGGGAGAGAAACTCTCAAAAACGGGAAAA460.25477707006369427No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA430.2381611741899751No Hit
ATTGCGCAATGTGGGAGCAGGGAGAATACTTCCAACAGTTGAGGTGTTAC410.22708391027416228No Hit
ATTGCGCAATGGAAGCAATGGGAGAAACTAAAAAAAGCAAAATATAACAA410.22708391027416228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.22154527831625587No Hit
ATTGCGCAATGGAGCGTACGGGATGGTCTCACATCCACAGGTTCATCCAG370.20492938244253667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT370.20492938244253667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA370.20492938244253667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT350.1938521185267239No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC310.17169759069509832No Hit
ATTGCGCAATGAACCACCTGGGAGGAGATACCCATGAAATCAACTCTGTT310.17169759069509832No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG300.16615895873719191No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.16062032677928553No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC290.16062032677928553No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA270.14954306286347271No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCT260.14400443090556633No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGA250.13846579894765992No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.13846579894765992No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAAGCG250.13846579894765992No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.13846579894765992No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT240.13292716698975354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.13292716698975354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG230.12738853503184713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.12738853503184713No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC220.12184990307394074No Hit
ATTGCGCAATGAGCTGGACGGGAGAAACTAAAAAAAGCAAAATATAACAA210.11631127111603433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT190.10523400720022155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)