FastQCFastQC Report
Tue 7 May 2024
EGAF00007823384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983
Total Bases601.4 kbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT40010.042681395932714No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1874.694953552598544No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC401.0042681395932713No Hit
GATTTATTTCTCGTTCCATCCTTGTCCTCATCCTCTCGCACAGTATTATC210.5272407732864675No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA200.5021340697966357No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT200.5021340697966357No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.3263871453678132No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC130.3263871453678132Illumina RNA PCR Primer (95% over 23bp)
AGATTTATTTCTCGTTCCATCCTTGTCCTCATCCTCTCGCACAGTATTAT130.3263871453678132No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3012804418779814No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAG120.3012804418779814No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT120.3012804418779814No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.25106703489831783No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA100.25106703489831783No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTC100.25106703489831783No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACG100.25106703489831783No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTG80.20085362791865427No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG80.20085362791865427No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA80.20085362791865427No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTG80.20085362791865427No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA80.20085362791865427No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGG80.20085362791865427No Hit
GTATTATCCGCCCGACCATCCCGTTTGTTCCCACTATTACATACTTATCC70.17574692442882248No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTCGGGGGGGGGG70.17574692442882248No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGG70.17574692442882248No Hit
ATTACATACTTATCCCGTCCTTACCCTCACAAACTACCTGATGCACCCAC70.17574692442882248No Hit
CTATTACATACTTATCCCGTCCTTACCCTCACAAACTACCTGATGCACCC60.1506402209389907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG60.1506402209389907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.1506402209389907No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGG60.1506402209389907No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGGGGGGGGGG60.1506402209389907No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGG60.1506402209389907No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTGGTGGGGGGGGGGG50.12553351744915892No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGGGGGGGGGGGG50.12553351744915892No Hit
TACTTATCCCGTCCTTACCCTCACAAACTACCTGATGCACCCACCTATTG50.12553351744915892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC50.12553351744915892No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTATATCTCGGTGGTCGCC50.12553351744915892Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA50.12553351744915892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA50.12553351744915892No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTC50.12553351744915892No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12553351744915892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC50.12553351744915892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC50.12553351744915892No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA40.10042681395932714No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTCGGGGGGGGGG40.10042681395932714No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG40.10042681395932714No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGC40.10042681395932714No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAAT40.10042681395932714No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG40.10042681395932714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC40.10042681395932714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA40.10042681395932714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC40.10042681395932714No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAATATTT40.10042681395932714No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA40.10042681395932714No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT40.10042681395932714No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGG40.10042681395932714No Hit
ATTAGTACCAGTAGCCTTCACACCTTGCGGTGCGTCCAGGTCTGGCCTAT40.10042681395932714No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA40.10042681395932714No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGGGGGGG40.10042681395932714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)