FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG6323.7679604125678177No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCT4962.957133488344363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3722.217850116258272No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3011.7945507661122042No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2011.1983544983008405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1060.6319680438800452No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCT850.506766827639659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC790.4709950515709772No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTAT780.4650330888928635No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT720.42926131282418173No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAT640.38156561139927264No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC640.38156561139927264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT620.36964168604304537No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG480.28617420854945447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT450.2682883205151136No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA400.23847850712454538No Hit
ATTGCGCAATGCTGCGGAGGGGGAAATCTATTTAGGTAGAGCGTCCAGCG380.22655458176831814No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG370.2205926190902045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC370.2205926190902045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.21463065641209086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG330.19674476837774996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG310.18482084302152268No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.17885888034340905No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG280.16693495498718178No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAT270.16097299230906814No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA260.15501102963095453No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA250.14904906695284087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG240.14308710427472723No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC230.1371251415966136No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC220.13116317891849996No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA220.13116317891849996No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCGTATCG210.12520121624038635No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATG210.12520121624038635No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC200.11923925356227269No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTAGCG200.11923925356227269No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.11327729088415907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT190.11327729088415907No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCGCG180.10731532820604543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT180.10731532820604543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.10731532820604543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTA180.10731532820604543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT180.10731532820604543No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCC180.10731532820604543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.10135336552793181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)