FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTA12927.702855780122817No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3582.1343826387646816No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2511.4964526322065224No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGG2001.192392535622727No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1991.1864305729446134No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTC1290.769093185476659No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.6975496333392953No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGCC1140.6796637453049543Illumina Single End PCR Primer 1 (95% over 23bp)
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCA930.554462529064568No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTG780.4650330888928635No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.3875275740773863No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG620.36964168604304537No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT610.36367972336493176No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG600.3577177606868181No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC580.3457938353305908No Hit
ATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGG560.33386990997436355No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG540.3219459846181363No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3219459846181363No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG520.31002205926190907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA490.29213617122756813No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC400.23847850712454538No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2205926190902045No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG360.21463065641209086No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGGCGCC350.2086686937339772No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT310.18482084302152268No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGA310.18482084302152268No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA300.17885888034340905No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG290.1728969176652954No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC290.1728969176652954No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGGGTA280.16693495498718178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.16097299230906814No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAG270.16097299230906814No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA260.15501102963095453No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.15501102963095453No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGG260.15501102963095453No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC240.14308710427472723No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA230.1371251415966136No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGGC220.13116317891849996Illumina Single End PCR Primer 1 (95% over 21bp)
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA220.13116317891849996No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC220.13116317891849996No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA220.13116317891849996No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTC200.11923925356227269No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.11923925356227269No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTT200.11923925356227269No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATC190.11327729088415907No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11327729088415907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.11327729088415907No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA180.10731532820604543No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10731532820604543No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGGGGTCGCC170.10135336552793181No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA170.10135336552793181No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC170.10135336552793181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)