FastQCFastQC Report
Tue 7 May 2024
EGAF00007823391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7627
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG2643.4613871771338665No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCT2092.740264848564311No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1782.33381408155238No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1441.8880293693457455No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC911.193129670905992No Hit
ATTGCGCAATGTAGTCACAGGGGGGAGCCGAAGGGAAACCGAGTCTTAAC540.7080110135046545No Hit
ATTGCGCAATGATATATCCGGGAAGTTGTGTAGTGCGATCGGTGGATGCC420.5506752327258424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG420.5506752327258424No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGC410.5375639176609415No Hit
ATTGCGCAATGTTACACCAGGGCACCCACTGGAGGTCCGAACCGATTAAC400.5244526025960404No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC340.4457847122066343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC340.4457847122066343No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT330.4326733971417333No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.41956208207683227No Hit
ATTGCGCATGCTAAAACCGGGTGATGTGGTTCCCAAAGATGTCAATGCTG300.39333945194703024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT290.38022813688212925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT280.36711682181722827No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAT250.32778287662252525No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT230.3015602464927232No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA210.2753376163629212No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.2360036711682182No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.20978104103841613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.20978104103841613No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTG160.20978104103841613No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC150.19666972597351512No Hit
ATTGCGCAATGTTGACCACGGGTAAAACGTAAAACACAAAATTTTACAAA150.19666972597351512No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.19666972597351512No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.18355841090861413No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG130.17044709584371315No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT130.17044709584371315No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG120.1573357807788121No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.1442244657139111No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG100.1311131506490101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.1311131506490101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT100.1311131506490101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG90.1180018355841091No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCTC90.1180018355841091No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG90.1180018355841091No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC90.1180018355841091No Hit
ATTGCGCAATGTATGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAG80.10489052051920807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGCT80.10489052051920807No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACTCGTGAAGCGATCG80.10489052051920807No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG80.10489052051920807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)