FastQCFastQC Report
Tue 7 May 2024
EGAF00007823393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11360
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG4413.8820422535211265No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT3062.693661971830986No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1631.4348591549295775No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1441.267605633802817No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1010.8890845070422535No Hit
ATTGCGCAATGAATATCGCGGGAGACAAGAAATGCCCCTTCACTGGTAAC960.8450704225352111No Hit
ATTGCGCAATGCCTCAAGGGGGGACCTTGGACCTGGACAGGCGGTCCGCC730.6426056338028169No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG700.6161971830985915No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTC650.5721830985915494No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAT530.4665492957746479No Hit
ATTGCGCAATGGATTGCCGGGGGTGAATGCGCATGCCCAGCGCCGTATCG510.448943661971831No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC470.41373239436619713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.3785211267605634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT410.3609154929577465No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC410.3609154929577465No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG400.35211267605633806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT390.3433098591549296No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC350.30809859154929575No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG330.2904929577464789No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.22887323943661972No Hit
ATTGCGCAATGGAGAGCGAGGGGCCGCGTCTACTACCCGGCGCCGTACCT260.22887323943661972No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.21126760563380279No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC220.1936619718309859No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.17605633802816903No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG200.17605633802816903No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA190.16725352112676056No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA180.15845070422535212No Hit
ATTGCGCAATGGTGGAGCGGGGGGGAGCCCATGGTTGTGGGTGAGTGAGT180.15845070422535212No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCGCG180.15845070422535212No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.14964788732394366No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG170.14964788732394366No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.14084507042253522No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCG160.14084507042253522No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC150.13204225352112675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.13204225352112675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.13204225352112675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT150.13204225352112675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.13204225352112675No Hit
ATTGCGCAATGGAATTGGAGGGAGAATTTAACTATGGGGTAGCAGAACGT150.13204225352112675No Hit
ATTGCGCAATGCGATCTTGGGGCAGATTGGTGCCTAGGAACTACGGCGGA140.12323943661971831No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGG130.11443661971830986No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.11443661971830986No Hit
ATTGCGCAATGTTCAAGGCGGGGGGGAGCCCATGGTTGTGGGTGAGTGAG130.11443661971830986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.11443661971830986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT130.11443661971830986No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGAGTCGATCG120.10563380281690139No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG120.10563380281690139No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT120.10563380281690139No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCT120.10563380281690139No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT120.10563380281690139No Hit
ATTGCGCAATGACAACGGGGGGATCTGAGTTCAGACCGGAGTAATCCAGG120.10563380281690139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)