FastQCFastQC Report
Tue 7 May 2024
EGAF00007823394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11360
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTA8077.10387323943662No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2071.8221830985915493No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1401.232394366197183No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGG1050.9242957746478873No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.7130281690140845No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTC790.6954225352112676No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCA770.6778169014084506No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.6602112676056338No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGCC660.5809859154929577Illumina Single End PCR Primer 1 (95% over 23bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTG490.4313380281690141No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG420.36971830985915494No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.3609154929577465No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC380.3345070422535211No Hit
ATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGG360.31690140845070425No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG360.31690140845070425No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT350.30809859154929575No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA310.27288732394366194No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.23767605633802816No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGA270.23767605633802816No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.23767605633802816No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC260.22887323943661972No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.20246478873239435No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGGC210.18485915492957747Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG210.18485915492957747No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA210.18485915492957747No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.18485915492957747No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGGCGCC210.18485915492957747No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.17605633802816903No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.17605633802816903No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC200.17605633802816903No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA180.15845070422535212No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.14964788732394366No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGGGTA170.14964788732394366No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG170.14964788732394366No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGG170.14964788732394366No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.14964788732394366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.13204225352112675No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAG150.13204225352112675No Hit
GTCTTGCTCAGGGGCCTGCACTCTCCAACTGTGACAATGTCTCCGATCTG150.13204225352112675No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA150.13204225352112675No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT140.12323943661971831No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA140.12323943661971831No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA140.12323943661971831No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC130.11443661971830986No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA130.11443661971830986No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA130.11443661971830986No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT130.11443661971830986No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.10563380281690139No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTTAT120.10563380281690139No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT120.10563380281690139No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTGGCC120.10563380281690139No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)