FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16097
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCG6193.8454370379573835No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCT4172.590544822016525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2641.6400571535068644No Hit
ATTGCGCAATGCGTCAGCAGCTGGACATTTACGCATCGATGGTGTTCTGC2331.4474746847238615No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1911.1865565012114059No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1791.1120084487792756No Hit
ATTGCGCAATGCTTATACCGGGGTGCACAGGCGAGGGCGGGAACGGCGGA1681.043672734049823No Hit
ATTGCGCAATGCACGTCAGGGGGATGTTAACCCTCTTACGCTCGGTATCG1641.018823383239113No Hit
ATTGCGCAATGCCATTATGGGGCGTCCGGTATGATTATCCAGCTCCTTGC1590.9877616947257253No Hit
ATTGCGCAATGGCCGGCAGGGGCAAGTACGCGCCAGGACCGCTGGCAGCG1570.9753370193203702No Hit
ATTGCGCAATGGGGTACGGGGGCGTTCTTAGTTGGTGGAGTGATTTGTCT1100.6833571472945269No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGC970.6025967571597193No Hit
ATTGCGCAATGTGCGAGGGGGGCATGGAGCTTCGAAAACAGTGGTTTTCG940.5839597440516866No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG750.4659253277008138No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC690.4286513014847487No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT640.3975896129713611No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG630.3913772752686836No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAT610.3789525998633286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT580.360315586755296No Hit
ATTGCGCAATGATTTGGGGGGGGGCGCTTTGGCAGGCGGTCATGGCGGCG580.360315586755296No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT440.2733428589178108No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCG430.26713052121513325No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC420.26091818351245577No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT330.20500714418835805No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC330.20500714418835805No Hit
ATTGCGCAATGTCTTCGCAGGGGTGACGTATGCAAGGCATGCCAGTGCGT330.20500714418835805No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG290.180157793377648No Hit
ATTGCGCAATGTACGAGGAGGGTAGTCGTGCCGTCGGCGTGATTCGCGAG280.1739454556749705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.1739454556749705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC270.16773311797229296No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.16152078026961544No Hit
ATTGCGCAATGATGACACCGGGCTCAATAAAGCCGTCTGGGCCAAGGGAA260.16152078026961544No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG240.14909610486426042No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGG240.14909610486426042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT230.1428837671615829No Hit
ATTGCGCAATGAAGTTTTAGGGGATTGGTCAGGCTTTGAAGAGGTTCCAG230.1428837671615829No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT230.1428837671615829No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAGCG230.1428837671615829No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA220.1366714294589054No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT210.13045909175622789No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG210.13045909175622789No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.13045909175622789No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.12424675405355035No Hit
ATTGCGCAATGCGATGGGCGGGAAAACGCGAGGACAAGTATCGACAGAGG200.12424675405355035No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATGG180.11182207864819532No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.11182207864819532No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.11182207864819532No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACG180.11182207864819532No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.10560974094551781No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.10560974094551781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)