FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15770
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG7364.66708941027267No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT4983.1578947368421053No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1751.109701965757768No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1550.9828788839568802No Hit
ATTGCGCAATGAGAAAGGGGGGGGGCTTGTGGATGGCGTCACTGTGAACC1440.9131261889663919No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCT1190.7545973367152822No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1080.684844641724794No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1010.6404565630944832No Hit
ATTGCGCAATGATTACTAGGGGGGACGATGGGGAAGAAAAAGGGCGGTAG1000.6341154090044389No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAT880.5580215599239061No Hit
ATTGCGCAATGAAGAGGGAGGGGAAAAAGAGATTAAGAAAGGAGAAATAA770.48826886493341787No Hit
ATTGCGCAATGGACAGTACGGGGGCGTGAGGCCAGCCTGCTGGCGGTCGT700.4438807863031071No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC670.424857324032974No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC620.39315155358275206No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC610.3868103994927077No Hit
ATTGCGCAATGTTAGCCCAGGGAACTTTGACACTTTTTATATCCATATAT550.3487634749524413No Hit
ATTGCGCAATGCGATGTCGGGGCACGCGTAACTTCGGAAGAAGCGTGACC540.342422320862397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT500.31705770450221943No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.2853519340519975No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.2726696258719087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC390.24730500951173112No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC370.23462270133164237No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG350.22194039315155356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA330.2092580849714648No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG320.20291693088142043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG300.19023462270133165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT290.18389346861128728No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA290.18389346861128728No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAGCG280.17755231452124287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT280.17755231452124287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.15852885225110971No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT240.1521876981610653No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA230.14584654407102093No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT230.14584654407102093No Hit
ATTGCGCAATGCGATCAGAGGGGGCGTCCCCAGCGGCGCGGTAGCGCTAC220.13950538998097653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC220.13950538998097653No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGC220.13950538998097653No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC200.12682308180088775No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.12048192771084339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG190.12048192771084339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.12048192771084339No Hit
ATTGCGCAATGACATACACGGGGTCAGGAGTTCGAGACAAGCCTGGCCAA180.11414077362079898No Hit
ATTGCGCAATGGTTCTGTAGGGCTGATCATGTACTTAAGGAAGTTGCACC170.1077996195307546No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCTCG170.1077996195307546No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCGGATCG170.1077996195307546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT170.1077996195307546No Hit
ATTGCGCAATGAACTACTCGGGGGCCGGCAGCGAGGTCATGGGCGTGGAT160.10145846544071022No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG160.10145846544071022No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG160.10145846544071022No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT160.10145846544071022No Hit
ATTGCGCAATGCGCACCCCGGGGAGGAGAGGATAGAAATAGTGGAAATGA160.10145846544071022No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT160.10145846544071022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)