FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15770
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTA13628.636651870640456No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2031.2872542802790108No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGG1701.077996195307546No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1270.8053265694356373No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCA1270.8053265694356373No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.7545973367152822No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTC1070.6785034876347495No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGCC930.5897273303741281Illumina Single End PCR Primer 1 (95% over 23bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTG840.5326569435637286No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.5009511731135067No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG660.4185161699429296No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG610.3868103994927077No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT590.37412809131261887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG580.36778693722257455No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC530.33608116677235256No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA490.31071655041217505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG450.2853519340519975No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC400.2536461636017755No Hit
ATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGG390.24730500951173112No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG370.23462270133164237No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGA360.22828154724159797No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC350.22194039315155356No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.20291693088142043No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC290.18389346861128728No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA280.17755231452124287No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.1648700063411541No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.1648700063411541No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGGCGCC260.1648700063411541No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGGGGTCGCC250.15852885225110971No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.15852885225110971No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC250.15852885225110971No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA240.1521876981610653No Hit
GATTTAAGAGAAAGCCTTTTTGAGAAGTTTGCAAGTTCTTTCTGTATATT240.1521876981610653No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTC230.14584654407102093No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGG230.14584654407102093No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA220.13950538998097653No Hit
GTATTACAACTGAGCCATTAGTCGTGTCGCTTCATCAACGTTAGCTGGTT210.13316423589093215No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.13316423589093215No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT200.12682308180088775No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG200.12682308180088775No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC200.12682308180088775No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT190.12048192771084339No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA190.12048192771084339No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATC190.12048192771084339No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA180.11414077362079898No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.11414077362079898No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA180.11414077362079898No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC180.11414077362079898No Hit
GTCATGGAATGTGGATCATGGGACCTGGCCCTGCCTGGAGCACCAAGATC170.1077996195307546No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA170.1077996195307546No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA170.1077996195307546No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA160.10145846544071022No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGGGTA160.10145846544071022No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA160.10145846544071022No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT160.10145846544071022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)