FastQCFastQC Report
Tue 7 May 2024
EGAF00007823414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12438
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTA7956.39170284611674No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2481.9938896928766683No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1801.447178002894356No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1631.3105000803987779No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGG1240.9969448464383341No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTC850.6833896124778903No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCA800.6431902235086027No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGCC780.6271104679208876Illumina Single End PCR Primer 1 (95% over 23bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.602990834539315No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTG540.43415340086830684No Hit
ATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGG480.3859141341051616No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG460.3698343785174465No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.32963498954815884No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG360.2894356005788712No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT350.2813957227850136No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.25727608940344104No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG290.23315645602186846No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGGCGCC280.22511657822801096No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.21707670043415342No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.21707670043415342No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.21707670043415342No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.20099694484643832No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGA250.20099694484643832No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.20099694484643832No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC240.1929570670525808No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTCGG240.1929570670525808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC230.18491718925872325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.17687731146486574No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC210.1688374336710082No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG210.1688374336710082No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGTGGTCGGC200.16079755587715067Illumina Single End PCR Primer 1 (95% over 21bp)
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC200.16079755587715067No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.15275767808329313No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.15275767808329313No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGTTCTC180.1447178002894356No Hit
CATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATCTCGGGGGTCGCC180.1447178002894356No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTC180.1447178002894356No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.1447178002894356No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAG160.12863804470172052No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.12863804470172052No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT160.12863804470172052No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA160.12863804470172052No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG150.120598166907863No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA130.10451841132014794No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGG130.10451841132014794No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA130.10451841132014794No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA130.10451841132014794No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATAAGGTGTCAGTGTAGATC130.10451841132014794No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10451841132014794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)