FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5259
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG2354.468530138809659No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCT1512.871268301958547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1202.2818026240730176No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC861.6352918805856627No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC551.045826202700133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG390.7415858528237307No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGC300.5704506560182544No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT210.3993154592127781No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT200.38030043734550295No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAT200.38030043734550295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC170.32325537174367747No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.2852253280091272No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA140.2662103061418521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG140.2662103061418521No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG120.22818026240730174No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG110.2091652405400266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.2091652405400266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC100.19015021867275148No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG100.19015021867275148No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT90.17113519680547634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC90.17113519680547634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT90.17113519680547634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT90.17113519680547634No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA90.17113519680547634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT90.17113519680547634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC80.15212017493820118No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC80.15212017493820118No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.13310515307092605No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCT70.13310515307092605No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACTCGTGAAGCGATCG70.13310515307092605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT70.13310515307092605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG60.11409013120365087No Hit
ATTGCGCAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGA60.11409013120365087No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCTCG60.11409013120365087No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATGG60.11409013120365087No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA60.11409013120365087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)