FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5259
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTA1803.4227039361095266No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1152.186727514736642No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTT941.787412055523864No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGGA771.4641566837801865No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC691.3120365088419852No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT631.1979463776383343No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGGT611.1599163339037841No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.8176459402928313No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTAC360.6845407872219054No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC280.5324206122837042No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTG220.4183304810800532No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT200.38030043734550295No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC180.3422703936109527No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.32325537174367747No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.30424034987640236No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.2852253280091272No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.2662103061418521No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.2662103061418521No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.22818026240730174No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG120.22818026240730174No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA110.2091652405400266No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2091652405400266No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT100.19015021867275148No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC100.19015021867275148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA100.19015021867275148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC90.17113519680547634No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG90.17113519680547634No Hit
CATCTGACGCTGCCGACGACCAATGTTACGTGTAGATCTCGGTGGTCGCC90.17113519680547634Illumina RNA PCR Primer (100% over 23bp)
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA90.17113519680547634No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.17113519680547634No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA80.15212017493820118No Hit
CTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTAGATCTCGG80.15212017493820118No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA70.13310515307092605No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA70.13310515307092605No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG70.13310515307092605No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC70.13310515307092605No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAAT70.13310515307092605No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATG60.11409013120365087No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.11409013120365087No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTG60.11409013120365087No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.11409013120365087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.11409013120365087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGC60.11409013120365087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGA60.11409013120365087No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAA60.11409013120365087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG60.11409013120365087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA60.11409013120365087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)