FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9941
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTA3933.9533246152298562No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTT1821.8308017302082287No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGGA1731.740267578714415No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1601.6094960265566844No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1451.458605774066995No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGGT1111.1165878684236998No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.9254602152700936No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.7645106126144251No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTAC640.6437984106226737No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTACGTG580.5834423096267981No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT380.38225530630721255No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.34201790564329543No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.3118398551453576No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.3118398551453576No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA270.2716024544814405No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.2716024544814405No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.25148375414948193No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA240.24142440398350265No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.21124635348556484No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG210.21124635348556484No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.21124635348556484No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC200.20118700331958556No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.20118700331958556No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC190.19112765315360628No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG190.19112765315360628No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.181068302987627No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.181068302987627No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.17100895282164771No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC160.16094960265566843No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAATGTTA150.15089025248968918No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.15089025248968918No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC150.15089025248968918No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA140.14083090232370987No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC140.14083090232370987No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAT130.13077155215773062No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA130.13077155215773062No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.13077155215773062No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.12071220199175133No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA120.12071220199175133No Hit
CTTATACACATCTGACGCTGCCGACGACCAATGTTACGTGTAGATCTCGG120.12071220199175133No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA120.12071220199175133No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA120.12071220199175133No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA110.11065285182577204No Hit
GATCCGTAAATCTATTGGAAATGGAAATCAGTGCTATACAAAGAATCTTC110.11065285182577204No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA110.11065285182577204No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAA100.10059350165979278No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA100.10059350165979278No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA100.10059350165979278No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGA100.10059350165979278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)