FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15384
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG5623.653146125845034No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCT4142.691107644305772No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4042.626105044201768No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2651.7225689027561102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2301.4950598023920956No Hit
ATTGCGCAATGCGAGTCGGGGGGTCCGTCAAACTAACCTCACAGCAGACA1871.215548621944878No Hit
ATTGCGCAATGGTTGTCACGGGGTAGCAATCATTGTTATAGGAATTGTTG1390.9035361414456579No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG880.5720228809152366No Hit
ATTGCGCAATGGAGAGAGGGGGTAGGGGGTGTGGGATGGATAGAGGAAAG820.533021320852834No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTA810.5265210608424337No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC740.48101924076963076No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC680.4420176807072283No Hit
ATTGCGCAATGAATAGCTGGGGGCTCTTCGCCATCTTTACCCCGGCAGGC660.4290171606864275No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATAT650.42251690067602704No Hit
ATTGCGCGAAGGAGAGAGGGGGTAGGGGGTGTGGGATGGATAGAGGAAAG620.4030161206448258No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC610.3965158606344254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT530.34451378055122206No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT530.34451378055122206No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.30551222048881954No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA450.2925117004680187No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA450.2925117004680187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC440.2860114404576183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT390.25351014040561626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.2470098803952158No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.22100884035361415No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.22100884035361415No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.2015080603224129No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCA300.19500780031201248No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC290.18850754030161207No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.1690067602704108No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG250.1625065002600104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT250.1625065002600104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT240.15600624024961No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.14950598023920958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC210.13650546021840873No Hit
ATTGCGCAATGCGGTACGTGGGGTGACAAGCAACGAAAACCTCGAGTAGG200.13000520020800832No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.13000520020800832No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.1235049401976079No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.1235049401976079No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC190.1235049401976079No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCT190.1235049401976079No Hit
ATTGCGCAATGCGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAG180.1170046801872075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT170.11050442017680707No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.11050442017680707No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT160.10400416016640666No Hit
ATTGCACAATGCAGACACCGGGCCTGTGGTTGAGACAGCAGAGAAGTCGT160.10400416016640666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)