FastQCFastQC Report
Tue 7 May 2024
EGAF00007823441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12217
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG4994.084472456413195No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT3292.6929688139477777No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2502.0463288859785544No Hit
ATTGCGCAATGTGAAGGACGGGCAAGCAGGGTTTCTGAAAGAGATGCCAA2071.6943603175902429No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2011.645248424326758No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1741.424244904641074No Hit
ATTGCGCAATGATAAGAGAGGGGAAGCTATTCATCATTTGTTAGATATAC1511.2359826471310469No Hit
ATTGCGCAATGATCGACCCGGGCAGCCAAGGTGTTGGAGCAGCTCACAGG1040.8512728165670786No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCT740.6057133502496521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG600.49111893263485307No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAT590.48293361709093885No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC500.4092657771957109No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC470.38470983056396824No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.35196856838831136No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG410.33559793730048293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC400.3274126217565687No Hit
ATTGCGCAATGCTGAAGACGGGGAAGACAGTATTAACGAGCTTGGTCATT330.2701154129491692No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT310.25374478186134075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT310.25374478186134075No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA310.25374478186134075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG290.2373741507735123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.20463288859785544No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC230.188262257510027No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA210.17189162642219857No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.16370631087828436No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.16370631087828436No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.14733567979045592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT170.1391503642465417No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCG170.1391503642465417No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAGCG150.12277973315871327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.12277973315871327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.12277973315871327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC150.12277973315871327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.11459441761479906No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC130.10640910207088483No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.10640910207088483No Hit
ATTGCGCAATGATTCTCAAGGGCCAAGGAATATAGCCGGAAGAAAGGATA130.10640910207088483No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT130.10640910207088483No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG130.10640910207088483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)