FastQCFastQC Report
Tue 7 May 2024
EGAF00007823443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6657
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCG2563.8455760853237195No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCT2253.3799008562415502No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1321.9828751689950428No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1001.502178158329578No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC731.0965900555805919No Hit
ATTGCGCAATGCTTCTACGGGGGGTGGCGGCGGTAACTCGAAGTCGGATG460.6910019528316058No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG380.5708277001652395No Hit
ATTGCGCAATGGACTGTCCGGGCAGGATGAACGCTGGCGGCGTGCCTAAT370.5558059185819438No Hit
ATTGCGCAATGGAGACGTCGAGCTGAGCGATCGCAGCGGAGAACGAGATG360.540784136998648No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCA350.5257623554153523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC330.4957187922487607No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAAT330.4957187922487607No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT270.405588102748986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.3905663211656903No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.3755445395823945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.33047919483250715No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA200.3004356316659156No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGT190.28541385008261977No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT190.28541385008261977No Hit
ATTGCGCAATGGTGAATCCGGGGGAGCGGCCGATGTCTGATTAGCTAGTT180.270392068499324No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.22532672374943669No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.2103049421661409No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.19528316058284514No Hit
ATTGCGCAATGGTCTAGACGGGGGCGGCTTTGGTGACTCTAGATAACCTC120.18026137899954936No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA120.18026137899954936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.16523959741625358No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCG110.16523959741625358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.16523959741625358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.1502178158329578No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC100.1502178158329578No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGT100.1502178158329578No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTG100.1502178158329578No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA100.1502178158329578No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATGG90.135196034249662No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTC90.135196034249662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG80.12017425266636624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG80.12017425266636624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG80.12017425266636624No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGC80.12017425266636624No Hit
ATTGCGCAATGCGGGGCCGGGCGTTCGATGAATATAAAAAATAGTATATT80.12017425266636624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC80.12017425266636624No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCTCG80.12017425266636624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC70.10515247108307045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT70.10515247108307045No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTCTGCAAATCGCG70.10515247108307045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG70.10515247108307045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG70.10515247108307045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT70.10515247108307045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)