FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13987
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCG4523.231572174161722No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCT3972.8383498963323084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2001.4298991921069564No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1821.3012082648173304No Hit
ATTGCGCAATGTCACTCGGGGGAGGATAGCAGAAGCTCGTATCAGTTTTA1390.9937799385143347No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1180.8436405233431042No Hit
ATTGCGCAATGCACTAGTAGGGAAGGGAAGCAAGACGGAACATAAGAACA1090.7792950596982913No Hit
ATTGCGCAATGTGTGGCGGGGGATGACTTACAGGTCAATTTTATTTGTTA1040.7435475798956174No Hit
ATTGCGCAATGACTTATAGGGGGGAGGAGTCCCGGAGCCGAGCTGGAGCT890.6363051404875957No Hit
ATTGCGCAATGGAGACCGGGGGGGATTTTGTTGTAATTGTTTAATCTTAG750.5362121970401087No Hit
ATTGCGCAATGTGGCTCTCGGGAAGGGTTCCAGGGTCAAGTTTGTCTGCC740.529062701079574No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGA730.521913205119039No Hit
ATTGCGCAATGAACAGACTGGGGAAGGTTGAGGCATGATGGGGAGCCCAT630.4504182455136913No Hit
ATTGCGCAATGGGTACACGGGGGTTGTGGGTGAGTGAGTGATCCTGTACT600.428969757632087No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAAT510.3646242939872739No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG460.32887681418459996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.30742832630299566No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC430.30742832630299566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC420.30027883034246083No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.2859798384213913No Hit
ATTGCGCAATGTTCGTACCGGGATGAAGTCTCTCAATAAACTCTGTGTCA400.2859798384213913No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC350.2502323586187174No Hit
ATTGCGCAATGGCTCACATGGGTAAGTTATCCGCCGAAGCTGTGGCAACC350.2502323586187174No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT300.2144848788160435No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCG290.2073353828555087No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA290.2073353828555087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.17873739901336955No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC210.15013941517123042No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC200.14298991921069565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.14298991921069565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA180.12869092728962608No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.11439193536855652No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGA150.10724243940802175No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.10724243940802175No Hit
ATTGCGCAATGCTTTGCTCGGGGTGGATAGGTAGAGTATACCGAGGCGAT140.10009294344748695No Hit
ATTGCGCAAGACGACTGAGGGAGGTAATGGCTCACCAAGGCGACGACGGG140.10009294344748695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.10009294344748695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)