FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12339
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5604.538455304319637No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG4913.9792527757516813No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3723.0148310235837585No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT3202.5934030310397924No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.599724450927952No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTT670.5429937596239566No Hit
ATTGCGCAATGCCTAATTCGGGATAGTGAGATCTCAGTCTCTACTAAAAA660.5348893751519572No Hit
ATTGCGCAATGCCTGACGGGGGCTCGGGTGTGACGATTGTTCCCCTCTTG660.5348893751519572No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG650.5267849906799579No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAT640.5186806062079585No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG590.47815868384796173No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC520.4214279925439663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.3728016857119702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.34038414782397275No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG390.3160709944079747No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC370.299862225463976No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT340.27554907204797796No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA320.25934030310397926No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACA310.2512359186319799No Hit
ATTGCGCAATGACTAACCGGGGGGCTCGCGTAGCACGGAGGCCAAGGCTA270.2188183807439825No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA270.2188183807439825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.21071399627198314No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT240.19450522732798445No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT230.1864008428559851No Hit
ATTGCGCAATGCCAAGACCGGGAAGGACAACAAGTCGCCTGACGATGCCG200.16208768943998703No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA200.16208768943998703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.16208768943998703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.15398330496798768No Hit
ATTGCGCAATGGTCATACGGGGGAGAATCACGCTCCAATGCAGGTCACTC180.14587892049598833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.14587892049598833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.13777453602398898No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGT170.13777453602398898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.12967015155198963No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.12156576707999028No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGGTAATCG140.11346138260799092No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT140.11346138260799092No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC140.11346138260799092No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAGCG140.11346138260799092No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT130.10535699813599157No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA130.10535699813599157No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCGCG130.10535699813599157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)