FastQCFastQC Report
Tue 7 May 2024
EGAF00007823459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7820
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG3404.3478260869565215No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT2573.286445012787724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1561.9948849104859334No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1091.39386189258312No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTC580.7416879795396419No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC530.6777493606138107No Hit
ATTGCGCAATGTGGTACCAGGGGAAGCATGAGAATGCAAGGTTATCCCAT470.6010230179028133No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAT380.4859335038363171No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT370.47314578005115093No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG300.3836317135549872No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC290.37084398976982097No Hit
ATTGCGCAATGGTCTGTGGGGGCGGAAGAGGGCTACTACGACAACGAAGA280.35805626598465473No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG280.35805626598465473No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG250.319693094629156No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.26854219948849106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT200.2557544757033248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT200.2557544757033248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.24296675191815856No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG170.21739130434782608No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA160.20460358056265981No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.20460358056265981No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.1918158567774936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA130.16624040920716113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.1534526854219949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.1534526854219949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG120.1534526854219949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT120.1534526854219949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG120.1534526854219949No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA120.1534526854219949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.14066496163682865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.14066496163682865No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCGCG110.14066496163682865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT110.14066496163682865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT110.14066496163682865No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT100.1278772378516624No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATGG100.1278772378516624No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATT100.1278772378516624No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCG100.1278772378516624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT100.1278772378516624No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGAGTCGATCG90.11508951406649616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT90.11508951406649616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG90.11508951406649616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT80.10230179028132991No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCTCG80.10230179028132991No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC80.10230179028132991No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC80.10230179028132991No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)