FastQCFastQC Report
Tue 7 May 2024
EGAF00007823466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9101
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGG4905.384023733655642No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1351.4833534776398198No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGT1341.4723656741017472No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC790.8680364795077463No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTG760.8350730688935281No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.8350730688935281No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.6263048016701461No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAG540.593341391055928No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTG510.5603779804417097No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGAT440.48346335567520055No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGGGGGGG430.4724755521371278No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGG400.4395121415229096No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG350.3845731238325459No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTT340.3735853202944731No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTA330.3625975167564004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.3625975167564004No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT330.3625975167564004No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.2856828919898912No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.2746950884518185No Hit
CTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGG250.2746950884518185No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG250.2746950884518185No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA240.26370728491374573No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT210.23074387429952753No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.23074387429952753No Hit
ATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGG190.20876826722338201No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGA190.20876826722338201No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGAT190.20876826722338201No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.20876826722338201No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGGGGGGGGGG190.20876826722338201No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGA180.1977804636853093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG170.18679266014723656No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.17580485660916384No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA150.16481705307109107No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.16481705307109107No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA150.16481705307109107No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.16481705307109107No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.15382924953301835No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGA140.15382924953301835No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGG130.1428414459949456No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGG120.13185364245687287No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC120.13185364245687287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC110.12086583891880014No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA110.12086583891880014No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGTAGATCTCGGTGGTCGCC110.12086583891880014Illumina RNA PCR Primer (95% over 23bp)
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC110.12086583891880014No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA110.12086583891880014No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.1098780353807274No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGGGGGGGGGG100.1098780353807274No Hit
CTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGG100.1098780353807274No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGG100.1098780353807274No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC100.1098780353807274No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA100.1098780353807274No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA100.1098780353807274No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.1098780353807274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)