FastQCFastQC Report
Tue 7 May 2024
EGAF00007823469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9649
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG4224.373510208311742No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT3123.2334956990361694No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1771.8343869831070576No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1131.1711058140739974No Hit
ATTGCGCAATGATTACACCGGGGGCAACCTGTGTGGTTGGGTAGGGGAGC910.9431029122188828No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC690.7151000103637682No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACT630.6529174007669188No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAT470.4870971085086538No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG470.4870971085086538No Hit
ATTGCGCAATGTGAATCCCGGGGAATTGACGGGGGCCTGCACAAGCGGTG430.44564203544408754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT410.42491449891180433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT390.4041869623795212No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG380.3938231941133796No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC360.3730956575810965No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC320.3316405845165302No Hit
ATTGCGCAATGGGTAAGTGGGGGAAGACAGAGTGATGCTGGACATACGAG280.290185511451964No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT230.23836667012125612No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT210.21763913358897294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.2072753653228314No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA190.1969115970566898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.1969115970566898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.1969115970566898No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC180.18654782879054824No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG180.18654782879054824No Hit
ATTGCGCAATGCTAGCCACGGGGCTTTTTTTTTGGTTTCCGTTCCTGACC160.1658202922582651No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.15545652399212354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC150.15545652399212354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.145092755725982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.145092755725982No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAGCG140.145092755725982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT130.13472898745984038No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG130.13472898745984038No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC120.12436521919369882No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT110.11400145092755726No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.11400145092755726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.11400145092755726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC100.1036376826614157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG100.1036376826614157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC100.1036376826614157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.1036376826614157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG100.1036376826614157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG100.1036376826614157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)