FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11948
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG4763.98393036491463No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT3613.0214261801138265No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2191.8329427519250083No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2111.7659859390693002No Hit
ATTGCGCAATGGACCTTCCGGGGGAAGCTTGTCATCAATGGAAATCCCAT2081.7408771342484097No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1511.2638098426514899No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAG860.7197857381988617No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.6695681285570807No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAT790.6611985269501173No Hit
ATTGCGCAATGAGAAAAGGGGGAGAGGAAGCAAAAGGGAAAGAATGGCTA660.5523937060595916No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.460328088382993No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC520.43521928356210243No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC500.4184800803481754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT500.4184800803481754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.3933712755272849No Hit
ATTGCGCAATGGCGGGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGC400.33478406427854035No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT360.3013056578506863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.3013056578506863No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG350.2929360562437228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.2510880482089053No Hit
ATTGCGCAATGCTAATACAGGGGGGCAATCTCGACGAGCGTGAGATTGCC300.2510880482089053No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.2343488449949782No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG280.2343488449949782No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC270.22597924338801473No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.21760964178105122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.21760964178105122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.21760964178105122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.19250083696016068No Hit
ATTGCGCAATGTGTTGAAGGGGAGCATCTCGGGGATTACCATGGTGCTGG210.1757616337462337No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC210.1757616337462337No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA200.16739203213927017No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.15902243053230666No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.14228322731837964No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCA170.14228322731837964No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.12554402410445265No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCGCG150.12554402410445265No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.12554402410445265No Hit
ATTGCGCAATGTAGGGTGAGGGGTGGTGACCATCGAAAGGAGCAAGAGCA140.1171744224974891No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT140.1171744224974891No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGACTCG140.1171744224974891No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG140.1171744224974891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.1171744224974891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.1171744224974891No Hit
ATTGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGA130.10880482089052561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC130.10880482089052561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT130.10880482089052561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT130.10880482089052561No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCT130.10880482089052561No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.10880482089052561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.10880482089052561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT130.10880482089052561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT130.10880482089052561No Hit
ATTGCGCAATGCAGCGTAGGGGGGGAGATGAGAAAGGAAAAAGAAGGGGA120.1004352192835621No Hit
ATTGCGCAATGGAGCCCAGGGGGGGTAGCGACTGTTTACCAAAAACACAG120.1004352192835621No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1004352192835621No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC120.1004352192835621No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT120.1004352192835621No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT120.1004352192835621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)