FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11948
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGG5774.829260127217944No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2592.167726816203549No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1861.5567458988952125No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGT1721.4395714763977234No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1201.0043521928356212No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTG800.6695681285570807No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAG800.6695681285570807No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTG730.610980917308336No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.5775025108804821No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGGGGGGGGGG590.493806494810847No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGGGG570.47706729159691996No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG540.45195848677602946No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT500.4184800803481754No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGAT490.4101104787412119No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTA460.38500167392032136No Hit
CTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGGG380.3180448610646133No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGTT360.3013056578506863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC340.2845664546367593No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA300.2510880482089053No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC280.2343488449949782No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG270.22597924338801473No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.22597924338801473No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.21760964178105122No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.2092400401740877No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.2092400401740877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.19250083696016068No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGAT220.18413123535319717No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.1757616337462337No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.1757616337462337No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC200.16739203213927017No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA190.15902243053230666No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGTGGGGG180.15065282892534315No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC180.15065282892534315No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA170.14228322731837964No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.14228322731837964No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGA170.14228322731837964No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGA150.12554402410445265No Hit
CATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGGGGGGGGGG150.12554402410445265No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.1171744224974891No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA140.1171744224974891No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGA130.10880482089052561No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA130.10880482089052561No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA130.10880482089052561No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10880482089052561No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAT120.1004352192835621No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.1004352192835621No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT120.1004352192835621No Hit
CTTATACACATCTGACGCTGCCGACGATCGATATGAGGTGGGGGGGTGGG120.1004352192835621No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.1004352192835621No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG120.1004352192835621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)