FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14429
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG4953.4305911705592904No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCT4763.2989119135075198No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2601.801926675445284No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2551.7672742393790284No Hit
ATTGCGCAATGATTCCGCTGGGTGTCCGGGGCTAAGTGTGTGGACTGTCT1661.1504608773996812No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1631.1296694157599279No Hit
ATTGCGCAATGACAGTTAGGGGTCAAACTTGAGAGTTTGATCCTGGCTCA1631.1296694157599279No Hit
ATTGCGCAATGTCTCGGCGGGGGATTGATGCTACTTGAGGATGGCCACAA960.6653267724721048No Hit
ATTGCGCAATGGTTTAGCCGGGGTAAATAAAATTTGAGAGGTATCAAAAT900.6237438491925982No Hit
ATTGCGCAATGTGTGGGTAGGGGCTCTCTCGAACTAGGCTGCTTCAGCCG890.6168133619793472No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCT810.5613694642733384No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG710.4920645921408275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.46434264328782315No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAAT620.4296902072215677No Hit
ATTGCGCAATGGAGGTAGGGGGGGATCGATCAGTTACGCAAGTAGGATAT580.4019682583685633No Hit
ATTGCGCAATGAAGTCATAGGGTAGAATATTGTATAACACATAGATTCCT580.4019682583685633No Hit
ATTGCGCAATGCCATTCACGGGGAGGGGAGTGAAACAGTACCTGAAACCG560.3881072839420611No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT560.3881072839420611No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC560.3881072839420611No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGC540.37424630951555893No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT440.304941437383048No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT410.28414997574329476No Hit
ATTGCGCAATGGTTGAGGGGGGAGGAAAAAATAAAAAAGGGAGAATGAAA410.28414997574329476No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.2772194885300437No Hit
ATTGCGCAATGTCCGAATTGGGATTCAAGATGTGGAAAAATATAAAGGCG380.26335851410354144No Hit
ATTGCGCAATGTAGTCATGGGGAAGGATGCACTGGCTTCTGCCGTCAAGG360.2494975396770393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.22177559082403492No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCG300.20791461639753273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.20098412918428166No Hit
ATTGCGCAATGAGACTACCGGGGGAATATAACGAATTGTATTTCTTCGAT280.19405364197103056No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGC270.18712315475777946No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA260.1801926675445284No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.1801926675445284No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.17326218033127727No Hit
ATTGCGCAATGGAGAGGGAGGGGAGAGATAAGGGTGAGTAAAAGAAGAGG240.1663316931180262No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC230.1594012059047751No Hit
ATTGCGCAATGAATTCGTCGGGCAGAAAGAATCTACTCTTCACCTTGTGC220.152470718691524No Hit
ATTGCGCAATGACTCGTTGGGGGCGAGGAAGGAGAAAAAGAAAGAGTGAA220.152470718691524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA210.1455402314782729No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.1455402314782729No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.13860974426502184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.13860974426502184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.13860974426502184No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCT190.13167925705177072No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG190.13167925705177072No Hit
ATTGCGCAATGACTAGACAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGT190.13167925705177072No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAAGCG180.12474876983851965No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCA170.11781828262526854No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGC170.11781828262526854No Hit
ATTGCGCAATGGTTTGGAGGGGAAGTTCGGCAGCGGTGACATCCTGAAGA160.11088779541201746No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.11088779541201746No Hit
ATTGCGCAATGCCTGTTTGGGGATGGGGACACTCTGCTGCCAGGCGCGCT160.11088779541201746No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCAAGCTAATCGCG160.11088779541201746No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.11088779541201746No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGCA150.10395730819876636No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC150.10395730819876636No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG150.10395730819876636No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.10395730819876636No Hit
ATTGCGCAATGTTAAGTCCGGGGGGTCAAGTGAATAAGTGCATATGGTGG150.10395730819876636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)