FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14429
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGG5804.019682583685634No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3372.335574190865618No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2041.4138193915032227No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGT1531.060364543627417No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1461.0118511331346594No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.7762145678841222No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTG850.5890914131263427No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTT790.5475084898468362No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGGGGGGG650.450481668861321No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGG590.40889874558181444No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC580.4019682583685633No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTA560.3881072839420611No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTG530.36731582230230786No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT520.3603853350890568No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGG490.3395938734493035No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTT480.3326633862360524No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC480.3326633862360524No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG450.3118719245962991No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.3118719245962991No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.27028900131679257No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGG360.2494975396770393No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT320.22177559082403492No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.22177559082403492No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.22177559082403492No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA300.20791461639753273No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.20791461639753273No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA290.20098412918428166No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.19405364197103056No Hit
ATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGG280.19405364197103056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.18712315475777946No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC260.1801926675445284No Hit
CCTTTTGGGATCTTAGTTTCCTTATGAATATTTGAGCTTTTTAGTATGTA240.1663316931180262No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.1594012059047751No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT220.152470718691524No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.13860974426502184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.13860974426502184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC190.13167925705177072No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA170.11781828262526854No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT160.11088779541201746No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAT160.11088779541201746No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGTGGGGGGGGGGGGG160.11088779541201746No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC160.11088779541201746No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGA150.10395730819876636No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTGGG150.10395730819876636No Hit
GCTAAGGATCGTCGCCTTGGTAGGCTTTTACCCCACCAACTAGCTAATCC150.10395730819876636No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGT150.10395730819876636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)