FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12427
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCT4883.92693329041603No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCG4803.862557334835439No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2582.0761245674740483No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2201.770338778466243No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1341.0782972559748933No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCT840.6759475335962017No Hit
ATTGCGCAGTGGAGGCGACGGGGTCAACTCGGAGGAAGGTGGGGATGACG820.6598535447010542No Hit
ATTGCGCAATGGTTTCGAAGGGGGGGGGAGGGAAAAAGAAGAAAAGAGGA810.6518065502534803No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.6437595558059065No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.5391486279874467No Hit
ATTGCGCAGTGGAGACCGCCGGGGTCAACTCGGAGGAAGGTGGGGATGAC670.5391486279874467No Hit
ATTGCGCAATGGGATTAACGGGGAAAGGGGAGGGGAAAAGGGAATAAGTA590.474772672406856No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAT580.4667256779592822No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT550.44258469461656075No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCA550.44258469461656075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT530.426490705721413No Hit
ATTGCGCAATGTCGGATAGGGGGCCGAAAAAAAGGGTGCTAAAAGAAAAC420.33797376679810087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.28969180011265794No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCG350.2816448056650841No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG350.2816448056650841No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.2575038223223626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.2575038223223626No Hit
ATTGCGCAATGTGGTAGAGGGGAAGATCTATCAAATTAATACCCCTATCA310.24945682787478876No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA300.24140983342721495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG290.2333628389796411No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC280.22531584453206727No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC270.21726885008449345No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT260.2092218556369196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.2092218556369196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG260.2092218556369196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT250.20117486118934577No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC240.19312786674177193No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.18508087229419812No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG220.17703387784662428No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGC210.16898688339905044No Hit
ATTGCGCAATGCAGTATACGGGCAGGAAATAGCCCCTCCATTATAGACCG210.16898688339905044No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCT200.16093988895147662No Hit
ATTGCGCAATGAGGACGGGGGGGTAGTGGTTACCCCGGGCTGGGCCCCTC200.16093988895147662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.16093988895147662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT200.16093988895147662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG190.15289289450390278No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.15289289450390278No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.15289289450390278No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAA170.13679890560875513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT160.1287519111611813No Hit
ATTGCGCAATGGTGACGACGGGGTCAACTCGGAGGAAGGTGGGGATGACG150.12070491671360747No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.12070491671360747No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG150.12070491671360747No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.11265792226603363No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAGCG140.11265792226603363No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.11265792226603363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT140.11265792226603363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.11265792226603363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT140.11265792226603363No Hit
ATTGCGCAATGGTAAGTGGGGCGAGTGAATCGTGGTGAAGGAACTCGGCA130.1046109278184598No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.1046109278184598No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCGCG130.1046109278184598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)