FastQCFastQC Report
Tue 7 May 2024
EGAF00007823499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15825
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCG6323.9936808846761456No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCT5153.2543443917851502No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3302.085308056872038No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2461.5545023696682465No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2001.263823064770932No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGC1080.6824644549763034No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG810.5118483412322274No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAT810.5118483412322274No Hit
ATTGCGCAATGAATACACCGGGGTAGTGGCGAGCGAAATGGGAAGAGCCT780.49289099526066354No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC750.47393364928909953No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT720.4549763033175356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.40442338072669826No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG550.34755134281200634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT550.34755134281200634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.2654028436018957No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC380.24012638230647712No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA370.23380726698262244No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA350.22116903633491314No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCG340.21484992101105846No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG330.2085308056872038No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.20221169036334913No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.17061611374407584No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT230.14533965244865718No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAGCG230.14533965244865718No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG220.13902053712480253No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCGATCG210.13270142180094785No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA200.1263823064770932No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAGCT180.1137440758293839No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG170.10742496050552923No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.10742496050552923No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACG170.10742496050552923No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT160.10110584518167456No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCGCG160.10110584518167456No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATGG160.10110584518167456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC160.10110584518167456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)