FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15825
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGG6884.347551342812006No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4302.7172195892575037No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3021.9083728278041074No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGT1751.1058451816745656No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTG1170.7393364928909952No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.688783570300158No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTT1060.669826224328594No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.5876777251184834No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC770.4865718799368089No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGGGGGGG690.43601895734597157No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTG680.4296998420221169No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGG640.40442338072669826No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTT620.39178515007898895No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT580.3665086887835703No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG540.3412322274881517No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGG530.334913112164297No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTA480.30331753554502366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.30331753554502366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC410.25908372827804105No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.25908372827804105No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC390.24644549763033177No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGG370.23380726698262244No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.2274881516587678No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT350.22116903633491314No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC340.21484992101105846No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC320.20221169036334913No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTGGG290.18325434439178515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.1769352290679305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.1579778830963665No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.15165876777251183No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC220.13902053712480253No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.13902053712480253No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC220.13902053712480253No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.13270142180094785No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA210.13270142180094785No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA200.1263823064770932No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC200.1263823064770932No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.1263823064770932No Hit
ATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGG200.1263823064770932No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG200.1263823064770932No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGT190.12006319115323856No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTGGGGGGGGGGG190.12006319115323856No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGA180.1137440758293839No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGGGGGGGGGGGGGGGGGGGGG180.1137440758293839No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT180.1137440758293839No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAT170.10742496050552923No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10742496050552923No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT160.10110584518167456No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCT160.10110584518167456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT160.10110584518167456No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGTAGATCTCGGTGGTCGCC160.10110584518167456Illumina RNA PCR Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)