FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG5694.578371419375603No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT4773.8381074991953654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2381.9150305761184423No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2291.8426134534921146No Hit
ATTGCGCAATGATTACTTGGGGGGAGCAAACAGGATTAGATACCCTGGTA1651.3276472481493402No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1561.2552301255230125No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACT910.7322175732217573No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG690.5551979401351786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC600.482780817508851No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.41841004184100417No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT510.41036369488252333No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAT470.37817830704859995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT410.32990022529771484No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG330.265529449629868No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC320.2574831026713872No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.24943675571290633No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG300.2413904087544255No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.18506598004505956No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG230.18506598004505956No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG220.1770196330865787No Hit
ATTGCGCAATGCGCGCTAGGGGCTCTTCCACAGGAGGCCTACACGCCGCC210.16897328612809784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.16092693916961698No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT200.16092693916961698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.16092693916961698No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC180.14483424525265529No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.14483424525265529No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTAC180.14483424525265529No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG170.13678789829417443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.13678789829417443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.13678789829417443No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCTCG170.13678789829417443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG160.1287415513356936No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCGCG160.1287415513356936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.1287415513356936No Hit
ATTGCGCAATGCTAAAACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG160.1287415513356936No Hit
ATTGCGCAATGCACGGGTTGGGCAATCCGTGCGGCACCCGTACCGCAAAC150.12069520437721275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC150.12069520437721275No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATGG150.12069520437721275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC150.12069520437721275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC140.11264885741873189No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.11264885741873189No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT140.11264885741873189No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.11264885741873189No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC130.10460251046025104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT130.10460251046025104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.10460251046025104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.10460251046025104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT130.10460251046025104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)