FastQCFastQC Report
Tue 7 May 2024
EGAF00007823503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18166
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG7304.018496091599691No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCT6353.4955411207750746No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4312.3725641307937906No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2771.5248265991412528No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2091.1505009358141582No Hit
ATTGCGCAATGTATGGTCCGGGGGGACGCAGTGAGGTAGCTCATCCCCGG1470.8092040074865133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1350.7431465374876143No Hit
ATTGCGCAATGCGGGCCCCGGGGAGATAAGATAAAGAGAATCAGAGTTGT1220.6715842783221402No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTA890.48992623582516787No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC880.484421446658593No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATAT870.4789166574920181No Hit
ATTGCGCAATGAGAAGCCCGGGATAGTTATGGGGGCGGAGTGATGGGCAG860.47341186832544313No Hit
ATTGCGCAATGGAAGCATGGGGTACCGCAAGGGAAAGATGAAAGACTAAT820.45139271165914346No Hit
ATTGCGCAATGCGCACGTTGGGCGAGAAGAAGGACCGCTACGACGATGCT770.42386876582626887No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT740.40735439832654413No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT710.39084003082681934No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC700.3853352416602444No Hit
ATTGCGCAATGTCGTAAGCGGGGGGGTGCCGGCTGCCTGCTGTCGAGATG700.3853352416602444No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT690.3798304524936695No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC600.3302873499944952No Hit
ATTGCGCAATGTAACCAAGGGGAAGGATAAAAAAAGTAAAGGAAAAGGTA500.275239458328746No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC430.23670593416272157No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT390.2146867774964219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG370.20367719916327207No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA370.20367719916327207No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG360.1981724099966971No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC350.1926676208301222No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC340.18716283166354727No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA340.18716283166354727No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG320.17615325333039744No Hit
ATTGCGCAATGAGAGGTGGGGGAAATGTCAAGGGGCGAGGCTATGACACC300.1651436749972476No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.15413409666409777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG280.15413409666409777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.14312451833094791No Hit
ATTGCGCAATGTTCTTATTGGGGGAGTTTTCCACGAAACACCGGAGAGCT250.137619729164373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.13211493999779808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG240.13211493999779808No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATAGCG230.12661015083122318No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC220.12110536166464825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG220.12110536166464825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT210.11560057249807333No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTCTATCG210.11560057249807333No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCT210.11560057249807333No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG200.11009578333149841No Hit
ATTGCGCAATGTCTACACAGGGCAAGCTCGCGTACTGTGTGTCGTGCGCC200.11009578333149841No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC200.11009578333149841No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCTCG200.11009578333149841No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.11009578333149841No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.10459099416492347No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.10459099416492347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)