FastQCFastQC Report
Tue 7 May 2024
EGAF00007823511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12788
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT4283.3468877072255236No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG4103.2061307475758527No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2562.0018767594619957No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2411.8845792930872691No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1711.33719111667188No Hit
ATTGCGCAATGTGGAAGAAGGGGCTCTTTGGCTGCGATCCGCCTACGAGT1401.094776352830779No Hit
ATTGCGCAATGTCGTTACCGGGGGATCAAGCGACTAAGTGCATATGGTGG1301.0165780419142947No Hit
ATTGCGCAATGAAAGGGGGGGGAAGAGAAAAGGAAGGGAGGAGAAGAAGG780.6099468251485768No Hit
ATTGCGCAATGCAAGGCAGGGGATACTTCAGTCTCCCAGGATTTTTCTCG780.6099468251485768No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC690.539568345323741No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG660.5161088520487958No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCT650.5082890209571473No Hit
ATTGCGCAATGTAAACAGTGGGGGTTACAAAAGAACTCACAAAAGAATGA580.4535502033156084No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAT570.44573037222396No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC540.4222708789490147No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.390991554582421No Hit
ATTGCGCAATGGAGGGCGGGGGATACTTCAGTCTCCCAGGATTTTTCTCG490.3831717234907726No Hit
ATTGCGCAATGTTGGCATCGGGGGATCAAGCGACTAAGTGCATATGGTGG470.3675320613074758No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.3597122302158274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA430.3362527369408821No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT420.3284329058492337No Hit
ATTGCGCAATGGGAAGGGCGGGGCTTGTGTTAACTAGTCAGAAAGAGGTG410.32061307475758527No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA400.31279324366593686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC370.2893337503909916No Hit
ATTGCGCAATGTCTGGCACGGGGGATCAAGCGACTAAGTGCATATGGTGG360.28151391929934316No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG310.24241476384110106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.2267751016578042No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA280.21895527056615577No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA250.1954957772912105No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT240.1876759461995621No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.1876759461995621No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.1798561151079137No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG230.1798561151079137No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCG210.16421645292461684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.15639662183296843No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG200.15639662183296843No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.15639662183296843No Hit
ATTGCGCAATGGTACAAGCGGGGGGTCAAGTGAATAAGTGCATATGGTGG200.15639662183296843No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC190.14857679074132No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTC190.14857679074132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.14857679074132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG190.14857679074132No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA180.14075695964967158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.14075695964967158No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCT180.14075695964967158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.13293712855802314No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCGCG160.12511729746637473No Hit
ATTGCGCAATGAGGGCGGCGGGCGTGGTGGCGGGTGCCTGTAGTCCCAGC160.12511729746637473No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.12511729746637473No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCT160.12511729746637473No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.12511729746637473No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.11729746637472631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.11729746637472631No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.11729746637472631No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATGTCTCATCT150.11729746637472631No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG150.11729746637472631No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAT150.11729746637472631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.11729746637472631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG140.10947763528307788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.10947763528307788No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATGTCTCATCG130.10165780419142947No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTG130.10165780419142947No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.10165780419142947No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC130.10165780419142947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)