FastQCFastQC Report
Tue 7 May 2024
EGAF00007823512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12788
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGG6134.793556459180482No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3252.5414451047857365No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2101.6421645292461682No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGT1601.2511729746637474No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.8758210822646231No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTG1040.8132624335314358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.781983109164842No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGGGGGGG860.6725054738817642No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTT730.5708476696903347No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC690.539568345323741No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTA650.5082890209571473No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTG630.4926493587738505No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGTT560.43791054113231154No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGG550.4300907100406631No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGG480.3753518923991242No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.3440725680325305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG410.32061307475758527No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.32061307475758527No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT380.29715358148264No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG360.28151391929934316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG360.28151391929934316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA350.27369408820769475No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC300.23459493274945262No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA300.23459493274945262No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC300.23459493274945262No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC290.2267751016578042No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.21113543947450736No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGG270.21113543947450736No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.21113543947450736No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.20331560838285895No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT260.20331560838285895No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATT250.1954957772912105No Hit
ATCACCTGCTACGGCCGGACTTTCCATTCCGTTCGACTATCTTGCATGCT210.16421645292461684No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.15639662183296843No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA200.15639662183296843No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCT180.14075695964967158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.14075695964967158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA180.14075695964967158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.13293712855802314No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGT170.13293712855802314No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC170.13293712855802314No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC170.13293712855802314No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAT160.12511729746637473No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.12511729746637473No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGGGGGGGGGGGGG150.11729746637472631No Hit
ATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGGGGGGGG150.11729746637472631No Hit
CATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGGGTGGGGGGGGGGG150.11729746637472631No Hit
CTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGTGGG140.10947763528307788No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT140.10947763528307788No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGG140.10947763528307788No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA140.10947763528307788No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA130.10165780419142947No Hit
GGTTCAGTTTGCTCTAATTAGAGGCATGTTTTTAGTTACTGTATTTCGGG130.10165780419142947No Hit
CTTATACACATCTGACGCTGCCGACGACCATTGTCTTGTGGGGGTGGGGG130.10165780419142947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)