FastQCFastQC Report
Tue 7 May 2024
EGAF00007823514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15793
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT9996.325587285506237No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC5793.6661812195276386No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTA3722.3554739441524726No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGT3011.9059076806179953No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGA2281.443677578674096No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTT2181.3803583866269866No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.9117963654783765No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGG1140.721838789337048No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC980.6205280820616729No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTG880.5572088900145634No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGA590.3735832330779459No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAA430.2722725258025708No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGG410.25960868739314885No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.25960868739314885No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTG380.240612929779016No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.240612929779016No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC370.2342810105743051No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2342810105743051No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.2152852529601722No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC310.19628949534603937No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG300.18995757614132844No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGA300.18995757614132844No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC290.1836256569366175No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1836256569366175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.1709618185271956No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.16462989932248465No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAG260.16462989932248465No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.15829798011777368No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.1456341417083518No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.13930222250364085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.1329703032989299No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.12663838409421896No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC200.12663838409421896No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA190.120306464889508No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC180.11397454568479706No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1076426264800861No Hit
CATCTGACGCTGCCGACGACAGGAAGAGGGTGTAGATCTCGGTGGTCGCC160.10131070727537517Illumina RNA PCR Primer (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)