FastQCFastQC Report
Tue 7 May 2024
EGAF00007823519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450
Total Bases218.9 kbp
Sequences flagged as poor quality0
Sequence length151
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1087.448275862068964No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC483.310344827586207No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG171.1724137931034484No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.6206896551724138No Hit
ATTGCGCAATGGGTCGGCGGGGGGTCAGGCCGTTCAGGACGGTCTGGTGG80.5517241379310345No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.41379310344827586No Hit
ATTGCGCAATAGCACCCGTGGGGAAAAACGTGAGAAATAAAAAGAGAAAG50.3448275862068966No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.3448275862068966No Hit
GGGCGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.20689655172413793No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCATATACACATCTCCGAGC30.20689655172413793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.20689655172413793No Hit
GCGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.20689655172413793No Hit
GGGCGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC20.13793103448275862RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAATCTGTCTCTTATACACCTGTCTCGTATACACATCTCCGAGC20.13793103448275862No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTATTATACACATCTCCGAGCCC20.13793103448275862No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA20.13793103448275862No Hit
ATTGCGCAATATGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20.13793103448275862No Hit
CGTCTGTTGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAA20.13793103448275862No Hit
ATTGCGCAATGATAATGTGGGGGTAAACGATGGATGCTAGCCGTCGGGCA20.13793103448275862No Hit
GGGGTAGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTACGAGC20.13793103448275862No Hit
GGGGTGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG20.13793103448275862No Hit
GGGGTAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GGGGGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GCCCGGGGGCCGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
ATTGCGCAATCTGTCTCATATACACCTGTCTCTTATACACATCTCCGAGC20.13793103448275862No Hit
ATTGCGCAATCTGTCTCTTATACACGTGTCTCTTATACACATCTCCGAGC20.13793103448275862No Hit
GGCCTCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GGGGGCTGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GGGGGCTGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)