FastQCFastQC Report
Tue 7 May 2024
EGAF00007823520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450
Total Bases218.9 kbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT16511.379310344827587No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC714.8965517241379315No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC261.7931034482758619No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGGGGGGG130.8965517241379309No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGG120.8275862068965517No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.7586206896551724No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGG90.6206896551724138No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGTAGATCTCGGTGGTCGCC90.6206896551724138Illumina RNA PCR Primer (95% over 22bp)
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTGGGGGGGGGGG90.6206896551724138No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGGGGGGGGGG80.5517241379310345No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGG70.4827586206896552No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGGGGG60.41379310344827586No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGGGGGGGGGG60.41379310344827586No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTG60.41379310344827586No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.41379310344827586No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGGGGGGGGGGGGG50.3448275862068966No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.3448275862068966No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.3448275862068966No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGG40.27586206896551724No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTCGGGGGGGGGG40.27586206896551724No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGGGGGGGGG40.27586206896551724No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGG40.27586206896551724No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGGGGGGGGGGGGG40.27586206896551724No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGG40.27586206896551724No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGGGGGGGGGGGGG30.20689655172413793No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTCGGGGGGGGGG30.20689655172413793No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGG30.20689655172413793No Hit
GATGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA30.20689655172413793No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGTGGGTGGGGGGG30.20689655172413793No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGGTGGGGGGGTGGGGGGGG30.20689655172413793No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGGGGGGGGGGGGGGG30.20689655172413793No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGGGGGGG30.20689655172413793No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGGGGGGG30.20689655172413793No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTGGGGGGGGGGG30.20689655172413793No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGGGG30.20689655172413793No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.20689655172413793No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGGGTGGGGGG30.20689655172413793No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGTGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTGGGGGGGGGGG20.13793103448275862No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGGGGG20.13793103448275862No Hit
GGTATAGCCAGATAAGTAGATTTGTTTAGAACCATTCTTGTGAAATACTT20.13793103448275862No Hit
TACCTCAACTATGATGATCTAATTTTTGGTGAATAATATACATGATTAGA20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGGGTCGGGGGGGGGG20.13793103448275862No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTCGGTGGGGGGC20.13793103448275862No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGGGGGGGGGGGGG20.13793103448275862No Hit
GGGGTCTCGGTTGGTTTGTCGGCGGTTTTTCCTTTTTTTTTCTTGTCCCT20.13793103448275862No Hit
GCTGTTGGCCTGTCTCCCTGGTCTGGTCTGGGCCCAGGGGTCTCGGTTGG20.13793103448275862No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTG20.13793103448275862No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTAGGGGGGGGGG20.13793103448275862No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.13793103448275862No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTTA20.13793103448275862No Hit
TCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGGGG20.13793103448275862No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTGGTTGTGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGTGGGGGGG20.13793103448275862No Hit
GTGTATAAGAGACAGATTGCGCAATGATCCCAGTGCCTCCATTGCGCAAT20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTCGGGGGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGCGGGGGGGGCG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGGGGTGGTGTGGGGGGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGTGGGGGGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGGGGGGGGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGGGGGGGGGGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTGGGTGGGGGGG20.13793103448275862No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTT20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGCGGGGGGGGGG20.13793103448275862No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTT20.13793103448275862No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTG20.13793103448275862No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTGGG20.13793103448275862No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTGGGGGGGGGGC20.13793103448275862No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACCACATCTGACGC20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGTGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGGGGGGC20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTCGGGGGGGGGG20.13793103448275862No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG20.13793103448275862No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGTGGGGGGGG20.13793103448275862No Hit
CATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGTGGGGGGGGGGGGGG20.13793103448275862No Hit
GTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC20.13793103448275862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)