FastQCFastQC Report
Tue 7 May 2024
EGAF00007823522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278
Total Bases343.9 kbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2229.745390693590869No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC823.5996488147497807No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241.0535557506584723No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC220.9657594381035997No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGG130.5706760316066725No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.4828797190517998No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGGGGGGGGGG90.39508340649692714No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGGGGGGG80.35118525021949076No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGG80.35118525021949076No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGGGGGGGGGG70.30728709394205445No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGG70.30728709394205445No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA70.30728709394205445No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTGGGGGGGGGGG60.2633889376646181No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGGGGG60.2633889376646181No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGG60.2633889376646181No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGTAGATCTCGGTGGTCGCC60.2633889376646181Illumina RNA PCR Primer (95% over 22bp)
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTGGGGGGGGGGG60.2633889376646181No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.2633889376646181No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTGGG60.2633889376646181No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGG50.21949078138718175No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGG50.21949078138718175No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTG50.21949078138718175No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.21949078138718175No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGTGGGGG40.17559262510974538No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGCGGGGGGGGGG40.17559262510974538No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGGGGGGGGGGGGG40.17559262510974538No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGG40.17559262510974538No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGT40.17559262510974538No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17559262510974538No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTT40.17559262510974538No Hit
GCCCTGCACCAGGGCCTACCTTGTTGCCAGGAAGCCGCACTGCTGGAGGC40.17559262510974538No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.17559262510974538No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17559262510974538No Hit
CCTTTCCAGGTTATTCTAATTCAATCCGCAACAAATGTCGTGGTCCTACA30.13169446883230904No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATTTA30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGTTGTTGTGGGGGGGGGGG30.13169446883230904No Hit
CTCCTCCACCCCGGGCTCGGCGGCACCATGCAGGCTCAGGCTGGCACTCA30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGGGGGGGGG30.13169446883230904No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTCGG30.13169446883230904No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGT30.13169446883230904No Hit
CCAGGAAACTGTCCCAGTTCTCAGCGGTCCTGGCTGTGGACGGTATCTGA30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGTGGGGGGGGGGG30.13169446883230904No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG30.13169446883230904No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTT30.13169446883230904No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC30.13169446883230904No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA30.13169446883230904No Hit
GTGTATAAGAGACAGAGTGCGCAATCTGTCTCTTATACACATCTGACGCT30.13169446883230904No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.13169446883230904No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTG30.13169446883230904No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC30.13169446883230904No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATGT30.13169446883230904No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTG30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGGGTGGGGGGGGGGG30.13169446883230904No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA30.13169446883230904No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGGGGGGGGGGGGG30.13169446883230904No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGGGGG30.13169446883230904No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGGGGGGGGGGGGG30.13169446883230904No Hit
CATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGTGGGGGGGGGGGGG30.13169446883230904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)