FastQCFastQC Report
Tue 7 May 2024
EGAF00007823523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025
Total Bases456.7 kbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC692.28099173553719No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA471.5537190082644627No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC391.2892561983471074No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG290.9586776859504132No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCT160.5289256198347108No Hit
ATTGCGCAATGTCGCGCGCGGGCAGCAAACCTTCCATGCTTCATTCCTCT130.4297520661157025No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGC110.36363636363636365No Hit
ATTGCGCAATGACACCGCGGGGGGGTCAAGTGAATAATTGCATATGGTGG100.3305785123966942No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.2644628099173554No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT80.2644628099173554No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAT70.23140495867768596No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG70.23140495867768596No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG70.23140495867768596No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT60.19834710743801653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT50.1652892561983471No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT50.1652892561983471No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG50.1652892561983471No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT40.1322314049586777No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT40.1322314049586777No Hit
ATTGCGCAATGTCTCGATGGGGGGGCCCATAGCTCAGTTGGTAGAGCAAC40.1322314049586777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC40.1322314049586777No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG40.1322314049586777No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT40.1322314049586777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)