FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT852.809917355371901No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461.5206611570247934No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC451.487603305785124No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.859504132231405No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGT250.8264462809917356No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGG200.6611570247933884No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTT120.39669421487603307No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG110.36363636363636365No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.36363636363636365No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.3305785123966942No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.3305785123966942No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.2975206611570248No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA90.2975206611570248No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.2644628099173554No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC80.2644628099173554No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTT70.23140495867768596No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA70.23140495867768596No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.23140495867768596No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC70.23140495867768596No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTG60.19834710743801653No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGG60.19834710743801653No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG50.1652892561983471No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGGGGGGG50.1652892561983471No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.1322314049586777No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGTAGATCTCGGTGGTCGCC40.1322314049586777Illumina RNA PCR Primer (95% over 22bp)
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1322314049586777No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC40.1322314049586777No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTGGGGGGGGGGG40.1322314049586777No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA40.1322314049586777No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGGGGGGGGGG40.1322314049586777No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA40.1322314049586777No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGG40.1322314049586777No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA40.1322314049586777No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC40.1322314049586777No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA40.1322314049586777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)