FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7246
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2483.422577974054651No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGT1572.166712669058791No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGG1411.9459011868617169No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1381.9044990339497654No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT931.283466740270494No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.7452387524151256No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTG400.5520287054926856No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA390.5382279878553685No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTT380.5244272702180514No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTT350.4830251173060999No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTG310.4278222467568314No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTA290.40022081148219707No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.40022081148219707No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG280.38642009384487996No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC260.3588186585702457No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.34501794093292854No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG250.34501794093292854No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.3174165056582942No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.3036157880209771No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC210.28981507038365995No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGGTGTGGG160.22081148219707425No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC140.19321004692243998No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG130.17940932928512285No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGT130.17940932928512285No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.17940932928512285No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT120.1656086116478057No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC120.1656086116478057No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1656086116478057No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGGTGTGGT110.15180789401048855No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC110.15180789401048855No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGA100.1380071763731714No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA100.1380071763731714No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGG90.12420645873585426No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGG80.11040574109853712No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA80.11040574109853712No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA80.11040574109853712No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT80.11040574109853712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)