FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2449.744408945686901No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1044.15335463258786No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC291.15814696485623No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.8386581469648562No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGG150.5990415335463258No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGT140.5591054313099041No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.43929712460063897No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGG110.43929712460063897No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGGGGGGGGGG110.43929712460063897No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGGGGGGG100.3993610223642172No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGG90.35942492012779553No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.35942492012779553No Hit
GCTCAGCACTGTGCACATGTGCGAAGAAGCCAGAGTAAGGCACTATGCTC80.3194888178913738No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGTAGATCTCGGTGGTCGCC80.3194888178913738Illumina RNA PCR Primer (95% over 22bp)
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGGGGGGGGGG70.27955271565495204No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTGGG60.23961661341853036No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTT60.23961661341853036No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGGGGGGGGGG60.23961661341853036No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC60.23961661341853036No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT60.23961661341853036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.1996805111821086No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGTGGGGGGGGGGG50.1996805111821086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG50.1996805111821086No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTG50.1996805111821086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA50.1996805111821086No Hit
GCCTTTACCTCACCAACTAGCTAATCCAACGCGGGCCGATCCTTTGCCGA50.1996805111821086No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA50.1996805111821086No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGGGGGGGGG40.1597444089456869No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGGGGGGGG40.1597444089456869No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTGGG40.1597444089456869No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC40.1597444089456869No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1597444089456869No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGGGGG40.1597444089456869No Hit
GATAAATACAGTATTACATAGAGTCATCGTACACCAACAAACGCCAGCGG40.1597444089456869No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGTAGATCTCGG40.1597444089456869No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGGGTG30.11980830670926518No Hit
CAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG30.11980830670926518No Hit
ATACAGTATTACATAGAGTCATCGTACACCAACAAACGCCAGCGGTTTCC30.11980830670926518No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA30.11980830670926518No Hit
GTATTAGCACAAGTTTCCCTGAGTTATTCCGTAGCAAAAGGTACGTTCCC30.11980830670926518No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG30.11980830670926518No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGA30.11980830670926518No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11980830670926518No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATGTGTG30.11980830670926518No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG30.11980830670926518No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGGGGGGGGGG30.11980830670926518No Hit
GTGTATAAGAGACAGAGTGCGCAATCTGTCTCTTATACACATCTGACGCT30.11980830670926518No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGGTGTGGGGGG30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.11980830670926518No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTGGTGGGGGGGGGGG30.11980830670926518No Hit
CCCATGGACTGTCTCCCGTAATTGAACTTCTCAGTATTCGGAGTTTGCAA30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA30.11980830670926518No Hit
CCTTAGAGTTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGC30.11980830670926518No Hit
GTATTACATAGAGTCATCGTACACCAACAAACGCCAGCGGTTTCCCACGA30.11980830670926518No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGGGGG30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.11980830670926518No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGTGGGGGGGGGGGGG30.11980830670926518No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCT30.11980830670926518No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA30.11980830670926518No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11980830670926518No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTTGTG30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA30.11980830670926518No Hit
CTTATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGTGGTGGG30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC30.11980830670926518No Hit
ATACACATCTGACGCTGCCGACGAGCGTAATGTTGTGGGGTTGTGGGGGG30.11980830670926518No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGTA30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC30.11980830670926518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA30.11980830670926518No Hit
CATCTGACGCTGCCGACGAGCGTAATGGTGTGGGGGTGGGGGGGGGGGGG30.11980830670926518No Hit
CATCTGACGCTGCCGACGAGCGTAATGTTGTGGTGGGGTGGGGGGGGGGG30.11980830670926518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)