FastQCFastQC Report
Tue 7 May 2024
EGAF00007823547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12168
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5214.28172255095332No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG4964.076265614727153No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3372.769559500328731No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT3212.638067061143984No Hit
ATTGCGCAATGAACCAAGGGGGGTAAGCAGAACTGGCGATGCGGGATGAA1010.8300460223537146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.6903353057199211No Hit
ATTGCGCAATGAATCTCTTGGGGGATGATCAGCCACACTGGGACTGAGAC780.641025641025641No Hit
ATTGCGCAATGGTTTCAGTGGGACCCACAGCAGCCGAGGCTGGCCATCTG740.6081525312294543No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.5506245890861275No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGG640.5259697567389875No Hit
ATTGCGCAATGGGGATACAGGGGGTTCCAGGAAATAGCCCCTCCATTATA630.5177514792899409No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT630.5177514792899409No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT610.5013149243918474No Hit
ATTGCGCAATGGTCGGGCGGGGGGTTCCAGGAAATAGCCCCTCCATTATA600.4930966469428008No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG580.47666009204470744No Hit
ATTGCGCAATGACATGCAGGGGGGATGTGCATATATGGTAGGAGAGCGTT540.4437869822485207No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAT540.4437869822485207No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC520.4273504273504274No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG460.3780407626561473No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG410.33694937541091385No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCA380.31229454306377386No Hit
ATTGCGCAATGGAAGCCCGGGGGAGGAATCCCGTGTGAATCTGCCAGGAC380.31229454306377386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG370.30407626561472717No Hit
ATTGCGCAATGCGTGCGGGGGGGATGTGCATATATGGTAGGAGAGCGTTC340.2794214332675871No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT340.2794214332675871No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC330.27120315581854043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.25476660092044706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.23833004602235372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC290.23833004602235372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT280.23011176857330704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.23011176857330704No Hit
CAGTGGGCTGGCAGCTTCCTGCTGCTCCCTGTCAGAGTCAAAGCACAAAT270.22189349112426035No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.22189349112426035No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAGCG260.2136752136752137No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT250.20545693622616698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG210.17258382642998027No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.1643655489809336No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.15614727153188693No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC180.14792899408284024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.13971071663379356No Hit
ATTGCGCAATGGGCTCGCGGGGATAATTGTCGGATGGCAAATCAACATAT160.13149243918474687No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT160.13149243918474687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT150.1232741617357002No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCGCG150.1232741617357002No Hit
ATTGCGCAATGGGCACTGGGGGACCCGAAACCAGGTGATCTACCCTTGGT150.1232741617357002No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT150.1232741617357002No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCTCG150.1232741617357002No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAC150.1232741617357002No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.1232741617357002No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT130.10683760683760685No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC130.10683760683760685No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT130.10683760683760685No Hit
ATTGCGCAATGTGGGGTGAGGGGGCCCGTTATCCGGGTCCGGGACCCTGC130.10683760683760685No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCG130.10683760683760685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)