FastQCFastQC Report
Tue 7 May 2024
EGAF00007823548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12168
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6655.465154503616041No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4293.5256410256410255No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTA2922.39973701512163No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGT2171.7833662064431293No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGA1721.413543721236029No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTT1691.3888888888888888No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGG600.4930966469428008No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC590.4848783694937541No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGA540.4437869822485207No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTG520.4273504273504274No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.3780407626561473No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG430.3533859303090072No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.33694937541091385No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC340.2794214332675871No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTG340.2794214332675871No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.2465483234714004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.22189349112426035No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.22189349112426035No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.2136752136752137No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGG250.20545693622616698No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.18080210387902695No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.17258382642998027No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAA200.1643655489809336No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA180.14792899408284024No Hit
GGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGG180.14792899408284024No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC180.14792899408284024No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG170.13971071663379356No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.13971071663379356No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13971071663379356No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTAGATCTCGG160.13149243918474687No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA160.13149243918474687No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13149243918474687No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA150.1232741617357002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.1232741617357002No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGG140.11505588428665352No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAG140.11505588428665352No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA140.11505588428665352No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.11505588428665352No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.10683760683760685No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT130.10683760683760685No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCT130.10683760683760685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)