FastQCFastQC Report
Tue 7 May 2024
EGAF00007823556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7835.7603178106378286No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4523.3252409328330756No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTA2631.9348193923342898No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTT1781.3094975354962113No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGT1721.2653571691311705No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGA1621.1917898918561023No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC780.5738247627455307No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTG640.47083057456043553No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.45611711910542196No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGG560.41197675274038104No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.38254984183035384No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGA500.3678363863753403No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG500.3678363863753403No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGG370.2721989259177518No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC320.23541528728021777No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.23541528728021777No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.22805855955271098No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAA310.22805855955271098No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.19863164864268373No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTG260.19127492091517692No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC230.1692047377326565No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC210.15449128227764292No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGG190.1397778268226293No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC190.1397778268226293No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.13242109909512248No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTAGATCTCGG180.13242109909512248No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG170.1250643713676157No Hit
CATCTGACGCTGCCGACGACAGGAAGAGGGTGTAGATCTCGGTGGTCGCC170.1250643713676157Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.1250643713676157No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1250643713676157No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.11770764364010888No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.11770764364010888No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA150.11035091591260207No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.11035091591260207No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAG150.11035091591260207No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.11035091591260207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)