FastQCFastQC Report
Tue 7 May 2024
EGAF00007823563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8229
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG3113.77931704945923No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2913.536274152387896No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT2302.7949933163203307No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1852.2481467979098313No Hit
ATTGCGCAATGCAGGCGAGGGGGGTCGGCCGTGTTGAGACTGGTGTGATC780.9478672985781991No Hit
ATTGCGCAATGGAACTAATGGGCGAGGACCTGTACCACCTTATCAAGCGC700.8506501397496659No Hit
ATTGCGCAATGATTGTAATGGGAGGAAGCGACTTGAGGATCCGCACCGAC700.8506501397496659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.6805201117997327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG430.5225422287033661No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAT380.46178150443553284No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT360.43747721472839957No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCG350.42532506987483293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC320.3888686353141329No Hit
ATTGCGCAATGCATTCTTGGGGGAATGATCATCGCTCAAGAAATAAAAGT310.37671649046056627No Hit
ATTGCGCAATGCGGAGGAGGGGCGTAAAGAACTTGCCTCACAGTTAGGGA280.34026005589986635No Hit
ATTGCGCAATGCGCGGGGTGGAGGCCCTGACTACCCTGGAAGTAGCAGGC280.34026005589986635No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT240.2916514764855997No Hit
ATTGCGCAATGTTTAAGACGGGGGCGTGAATGTGCACACAAACGCGAAGA230.27949933163203305No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT200.24304289707133309No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.24304289707133309No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG190.23089075221776642No Hit
ATTGCGCAATGGGTGGGGAGGGGGCCCTGACTACCCTGGAAGTAGCAGGC180.21873860736419978No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAGCG170.20658646251063315No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.19443431765706645No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.18228217280349981No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT140.17013002794993318No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC140.17013002794993318No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT130.1579778830963665No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCGCG130.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC130.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG130.1579778830963665No Hit
ATTGCGCAATGTAACACGGGGTGACGCCATCAAATCAGGTGTGGCAGACG130.1579778830963665No Hit
ATTGCGCAATGACTTCAACGGGGAACGGAAAAAAACAGAGAAAAAGAAGA130.1579778830963665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG120.14582573824279985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.14582573824279985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.14582573824279985No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC120.14582573824279985No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAGCT120.14582573824279985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT120.14582573824279985No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATG120.14582573824279985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC110.1336735933892332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT100.12152144853566654No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT100.12152144853566654No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTC90.10936930368209989No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCTCG90.10936930368209989No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)