FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10223
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4063.9714369558837914No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2692.6313215298835955No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTA2362.308520003912746No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGT1801.7607355962046365No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGA1581.5455345788907366No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTT1491.4574977990805047No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGG650.635821187518341No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.5086569500146728No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTG500.4890932211679546No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGGG420.4108383057810819No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.4108383057810819No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC410.4010564413577227No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.4010564413577227No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.3912745769343637No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG340.3325833903942091No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGA320.31301966154749095No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.28367406827741365No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTG270.2641103394306955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA270.2641103394306955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.20541915289054094No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC210.20541915289054094No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.19563728846718184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.15650983077374547No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.14672796635038637No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.14672796635038637No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.14672796635038637No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.14672796635038637No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGGAA140.13694610192702728No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.1271642375036682No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA130.1271642375036682No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAAGAGGGTGTAGATCTCGG120.1173823730803091No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA120.1173823730803091No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.1173823730803091No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.10760050865695002No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGG110.10760050865695002No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC110.10760050865695002No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC110.10760050865695002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)