FastQCFastQC Report
Tue 7 May 2024
EGAF00007823581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13051
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC7085.424871657344265No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4723.6165811048961762No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG4133.164508466784154No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT2762.1147804765918323No Hit
ATTGCGCAATGAGGTTCCGGGGGAGGAAGTGCTCGGGCGTCGCAGGGCCG1461.1186882231246646No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1441.1033637269174774No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG700.5363573672515516No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAAC660.5057083748371772No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTAT610.46739713431920926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG590.45207263811202203No Hit
ATTGCGCAATGACGTACGTGGGGGGTCAAGTGAATAAGAGCACGTGGTGG520.3984369013868669No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG470.36012566086889897No Hit
ATTGCGCAATGGTGGGGTGGGAAGATGCTAAAGAATGCAAAACTGATCCA460.35246341276530535No Hit
ATTGCGCAATGTCAATGTGGGGAGGTCCTGATTGATTACGTGGAAAAGCG450.34480116466171173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC420.321814420350931No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG390.2988276760401502No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT340.26051643552218223No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT330.2528541874185886No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGA300.22986744310780782No Hit
ATTGCGCAATGCCATTGTGGGGAGGTCCTGATTGATTACGTGGAAAAGCG260.19921845069343344No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT240.18389395448624626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.17623170638265268No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.16856945827905906No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG220.16856945827905906No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.13792046586468468No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT180.13792046586468468No Hit
ATTGCGCAATGGGTAGGCTGGGGCCGGGCGGGTTAGGCTACCGGGTGTAG170.13025821776109112No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG160.1225959696574975No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.1225959696574975No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC150.11493372155390391No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.11493372155390391No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA150.11493372155390391No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG140.10727147345031034No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA140.10727147345031034No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC140.10727147345031034No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.10727147345031034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)